TITLE 77: PUBLIC HEALTH
CHAPTER I: DEPARTMENT OF PUBLIC HEALTH
SUBCHAPTER d: LABORATORIES AND BLOOD BANKS
PART 465 CERTIFICATION AND OPERATION OF ENVIRONMENTAL LABORATORIES
SECTION 465.380 STANDARDS FOR LABORATORY PURE WATER


 

Section 465.380 Standards for Laboratory Pure Water

 

The following standards shall apply to all laboratory pure water:

 

a) Laboratory pure water shall have these characteristics:

 

Parameter

Limits

Frequency

Conductivity

>0.5 megohms resistance or <2 micromhos/cm at 25 C

Monthly

Cd, Cr, Cu, Pb, Ni, Zn

Not greater than 0.05 mg/L per contaminant. Collectively, no greater than 0.1 mg/L

Annually

Total Chlorine Residual 1

<0.1 mg/L

Monthly

Heterotrophic Plate Count 2

<500 CFU/mL

Monthly

Bacteriological Quality of Reagent Water 3

Ratio of growth rate 0.8 to 3.0

Annually

 

1 DPD Method shall be used. Not required if source water is not chlorinated.

 

2 Pour Plate Method. See Standard Methods 9215B. SimPlate method allowed with satisfactory comparison testing.

 

3 See Standard Methods, Section 9020B, under Laboratory Supplies. This bacteriological quality test is not needed for type II water or better, as defined in Standard Methods. If Type II or medium quality water or better is not available, and a glass still is used for reagent water, a silicon test that meets the specifications of Standard Methods, Section 1080C shall also be accomplished. The bacteriological quality test is not needed for water with a conductivity <1 micromhos/cm at 25 C or resistivity >1 megohms. Users of purchased bottled water are not exempt from the suitability test.

 

b) Laboratory pure water shall be analyzed initially and annually (every 12 months) thereafter by the test for bacteriological quality of distilled water as specified in "Standard Methods for the Examination of Water and Wastewater." Purchased laboratory pure water shall be sampled in-house; manufacturer's test results shall not be used to establish compliance. Only satisfactorily tested water shall be used in preparing media, reagents, rinse, and dilution water. If the water tested does not meet the testing requirements, the water shall not be used until corrective action has been taken and retesting determines that the testing requirements have been met.

 

c) Laboratory pure water shall be analyzed monthly for conductance, chlorine residual, and heterotrophic plate count. Heterotrophic plate counts shall be performed as specified in "Standard Methods for the Examination of Water and Wastewater." If the water tested exceeds requirements for these properties, the water shall not be used until corrective action has been taken and retesting determines that the testing requirements have been met.

 

d) Laboratory pure water shall not be in contact with heavy metals, and shall be analyzed initially and annually (every 12 months) thereafter for trace metals (especially Pb, Cd, Cr, Cu, Ni, and Zn) in the quantities specified in subsection (a) of this Section. If the water tested exceeds requirements for trace metals, the water shall not be used until corrective action has been taken and retesting determines that the testing requirements have been met.

 

(Source: Amended at 35 Ill. Reg. 14494, effective August 12, 2011)